Agent Check - Operations - Master: Connecting to the station

Unable to run the scenarios, as everytime I'm promted with "Agent check" and it waits for some 550+ sec and then timeout occurs.

Stations: Host - Localhost

Scenario recorder and simulated against - .Net web application

I had run more than 50 times the same scenarios against same application but never faced this issue in the past and currently I'm stuck with this issue. Please let me know if any additional info required for the analysis and a solution at the earlist is much appreciated